Module LS4027-KP06

Duration

1 Semester

Turnus of offer

each winter semester

Credit points

6

Course of studies, specific fields and terms:

• Master Infection Biology 2023, optional subject, life sciences
• Master Biophysics 2023, compulsory, biophysics
• Master Molecular Life Science 2023, optional subject, structure biology

Classes and lectures:

• NMR-Spectroscopy (lecture, 2 SWS)
• Single Molecule Methods (lecture, 2 SWS)

Workload:

• 120 hours private studies
• 60 hours in-classroom work

Contents of teaching:

• Lecture Singel Molecule Methods: Physical basics of fluorescence
• Photo physics
• Microscopy techniques
• Protein labeling
• Fluorescence resonance energy transfer
• Single molecule enzymology
• Single molecule protein folding
• Physical basics of optical tweezers
• Protein folding with optical tweezers
• NMR-Spectroscopy:
• Assignment of NMR spectra
• Description of the NOESY experiment using the vector model Chemical Exchange and Transfer-NOEs
• Multidimensional NMR spectroscopy
• Assignment strategy for peptides
• Introduction into the product operator formalism (POF)
• Description of the COSY and of the HSQC experiment using POF
• NMR experiments for the assignment of proteins
• NMR structural analysis of proteins
• Experiments to probe the motions of protein

Qualification-goals/Competencies:

• Lecture Singel Molecule Methods:
• Understanding of the physical basics of single molecule methods
• Understanding of the benefits of single molecule methods
• Understanding of the limits of single molecule methods
• Lecture NMR-Spectroscopy:
• Students are able to assign and analyze complex NMR spectra
• Understanding of NMR experiments based on the product operator formalism
• Students are able to analyze structure and dynamics of proteins through NMR experiments

Grading through:

• written exam

Responsible for this module:

• Prof. Dr. rer. nat. Ulrich Günther

Teacher:

• Institute of Chemistry and Metabolomics
• Institute of Physics
• Prof. Dr. rer. nat. Christian Hübner
• Prof. Dr. rer. nat. Ulrich Günther
• Dr. Alvaro Mallagaray

Literature:

• Lakowicz, Joseph R : Principles of Fluorescence Spectroscopy ISBN 978-0-387-46312-4
• Markus Sauer, Johan Hofkens, Jörg Enderlein : Handbook of Fluorescence Spectroscopy and Imaging: From Ensemble to Single Molecules ISBN: 978-3-527-31669-4
• James Keeler : Understanding NMR Spectroscopy
• Horst Friebolin : Ein- und zweidimensionale NMR-Spektroskopie. Eine Einführung
• Malcolm H. Levitt : Spin Dynamics - Basics of Nuclear Magnetic Resonance
• D. Neuhaus & M. P. Williamson : The Nuclear Overhauser Effect in Structural and Conformational Analysis

Language:

• offered only in English

Notes:

Last Updated:

08.11.2024