Module LS3251-KP05

Duration

1 Semester

Turnus of offer

each winter semester

Credit points

5

Course of studies, specific fields and terms:

• Bachelor Molecular Life Science 2024, optional subject, life sciences
• Bachelor Biophysics 2016, optional subject, life sciences

Classes and lectures:

• Tissue Engineering (seminar with practical exercises, 2 SWS)
• Tissue Engineering (lecture, 2 SWS)

Workload:

• 60 hours in-classroom work
• 90 hours private studies

Contents of teaching:

• Lectures: Mamalia cells in their natural environment and under in vitro culture as an example of industrial application.
• Aging of cells in vitro
• Established cell lines
• Diverse in vitro culturing conditions
• Proliferation and differentiation under in vitro conditions
• Stem cell biology
• Materials for medical applications an biotechnology
• Fermentors, bioreactors and protein purification
• Home work e. g. “Tissue transplantation and rejection”
• Practical course (in groups of 2): Principles of aseptic manipulations, working in sterile containments, object and selfprotection, use of autoclaves
• Preparation of sterile media, additives and other reagents
• Slicing of tissue samples, transfer into tissue culture flasks for explant cultures
• Microscopy and documentation of growing cells
• Cell count, passaging by trypsinisation
• Viability test, freezing of cells and reseeding after thawing
• Adherence of cells to various matrices
• Immunohistochemistry of intracellular and extracellular proteins

Qualification-goals/Competencies:

• Students are able to explain principles of cell- and tissue culture to generate biocomposites from differentiated and pluripotent cells
• They are able to explain basic principles of pro- and eukaryotic gene expression systems
• They are able to explain basic principles of organoid biology
• They can reproduce the aspects of stem cell biology
• They acquire the ability to assess ethical aspects of tissue engineering
• They improve their competence for correct documentation and team working skills

Grading through:

• written exam

Responsible for this module:

• Prof. Dr. rer. nat. Charli Kruse

Teacher:

• Institute of Medical and Marine Biotechnology
• Department of Dermatology, Allergology and Venerology
• Institute of Virology and Cell Biology
• Prof. Dr. rer. nat. Charli Kruse
• Dr. rer. nat. Daniel Hans Rapoport
• Dr. rer. nat. Philipp Ciba
• Prof. Dr. med. vet. Jennifer Hundt
• Prof. Dr. med. Ralf Ludwig
• Dr. rer. nat. Olaf Isken
• Dr. med. Dipl. Biol. Judith Sewing

Literature:

• Lanza, Langer, Vacanti : Principles of Tissue Engineering

Language:

• offered only in German

Notes:

Last Updated:

08.10.2024