Module BP4510-KP12

Duration

1 Semester

Turnus of offer

each summer semester

Credit points

12

Course of studies, specific fields and terms:

• Master Biophysics 2019, compulsory, biophysics
• Master Biophysics 2023, compulsory, biophysics

Classes and lectures:

• Proteinbiophysics (lecture, 2 SWS)
• Basics of Membrane Biophysics (lecture, 2 SWS)
• Basics of Membrane Biophysics (exercise, 1 SWS)
• Instrumentation in Biophysics (exercise, 1 SWS)
• Instrumentation in Biophysics (lecture, 2 SWS)
• Proteinbiophysics (exercise, 1 SWS)

Workload:

• 225 hours private studies
• 135 hours in-classroom work

Contents of teaching:

• Protein structure
• Energy landscapes
• Thermodynamics of protein folding
• Thermodynamics of protein folding
• Kinetics of protein folding
• Thermodynamics of enzymatic reactions
• Kinetics of enzymatic reactions
• Importance and function of cell membranes: structure, physical function and dynamic models
• Basics of the membrane components
• Thermodynamic self-assembling of lipids and reconstitution techniques
• Transmembrane and intrinsic membrane potentials
• Mechanical properties of lipid membranes
• Physical basics of membrane transport mechanisms
• Investigations using lipid monolayer
• Electrical and optical experiments using planar lipid bilayers
• Examples for interaction mechanisms between peptides/ proteins and planar membranes
• Spectroscopic methods on membranes and membrane proteins
• Light and force microscopy on membranes and membrane proteins
• UV-VIS spectroscopy
• Atomic force microscopy
• Fluorescence spectroscopy
• Film balance
• Patch clamp

Qualification-goals/Competencies:

• Students will understand and be able to apply the physical principles of protein folding, protein dynamics and protein interaction.
• They can name the components of biological membranes and explain their structure.
• They can name and explain the role and function of membrane lipids and proteins.
• They can name and explain the mechanical and electrical properties of membranes.
• They can select and apply appropriate methods for the investigation of artificial and natural membranes.
• They can identify the appropriate instrumentation for a particular question of biophysics.
• They can further develop instruments of biophysics
• They can use the instruments of biophysics optimally

Grading through:

• Oral examination

Requires:

• Introduction into Biophysics (LS2200-KP04, LS2200)

Responsible for this module:

• Prof. Dr. rer. nat. Christian Hübner

Teacher:

• Research Center Borstel, Leibniz Lung Center
• Institute of Physics
• Prof. Dr. rer. nat. Christian Hübner
• PD Dr. rer. nat. Hauke Paulsen
• Prof. Dr. rer. nat. Thomas Gutsmann
• Prof. Dr. rer. nat. Andra Schromm
• Dr. Christian Nehls

Literature:

• Hans Frauenfelder, Shirley Chan und Winnie Chan : Physics of Proteins: An Introduction to Molecular Biophysics (Biological and Medical Physics, Biomedical Engineering) von Springer, Berlin (Gebundene Ausgabe - 30. Dezember 2010)
• Alan Fersht : Structure & Mechanism in Protein Science: Guide to Enzyme Catalysis and Protein Folding W H Freeman & Co (Gebundene Ausgabe - 15. Februar 1999)
• Meyer B. Jackson : Molecular and Cellular Biophysics ISBN: 978-0-521-62470-1
• G. Adam, P. Läuger, G. Stark : Physikalische Chemie und Biophysik Springer-Verlag, 4. Auflage 2003
• W. Hanke, R. Hanke : Methoden der Membranphysiologie Spektrum Akademischer Verlag, Auflage 1997
• Ole G. Mouritsen : Life - As a Matter of Fat Springer 2005, ISBN 987-3-540-23248-3
• Thomas Heimburg : Thermal Biophysics of Membranes Whiley-VCH 2007, ISBN 978-3-527-40471-1
• Lukas K. Buehler : Cell Membranes Garland Science 2016, ISBN 978-0-8153-4196-3
• Yves Dufrene (Ed.) : Life at the Nanoscale Pan Stanford Publishing 2011, ISBN 978-981-4267-96-0

Language:

• German and English skills required

Last Updated:

02.11.2020