Module LS4137-KP09

Duration

1 Semester

Turnus of offer

every summer semester

Credit points

9

Course of studies, specific fields and terms:

• Master Molecular Life Science 2023, optional subject, life sciences

Classes and lectures:

• LS4139-S: Mass Spectrometry-based Proteomics and Structural Biology (seminar, 0.5 SWS)
• LS4137-V: Cryo-electron microscopy (lecture, 1 SWS)
• LS4138-S: Metabolomics (seminar, 1 SWS)
• LS4139-V: Mass Spectrometry-based Proteomics and Structural Biology (lecture, 1 SWS)
• LS4138-V: Metabolomics (lecture, 2 SWS)
• LS4137-S: Cryo-electron microscopy (seminar, 0.5 SWS)

Workload:

• 105 hours in-classroom work
• 165 hours private studies

Contents of teaching:

• +++Contents of teaching cryo-EM:+++
• Common procedures in the cryo-electron microscopy and application to biological systems
• Anatomy of the cryo-electron microscope
• Theoretical background: Fourier transforms and image formation
• Sample preparation
• Common procedures in the cryo-electron microscopy
• Single Particle Analysis (SPA) – Background and practical
• +++Contents of teaching Metabolomics:+++
• What is Metabolomics, how is it related to metabolism?
• Underlying analytical techniques: Mass spectrometry and NMR.
• Use of isotope labeling for metabolic flux analysis.
• Statistical methods used for Metabolomics.
• Computational flux-balance modelling.
• Case studies from current literature (pharmakometabolomics, role of metabolomics in drug research).
• +++Contents of Practicals Metabolomics:+++
• Metabolomics: Introduction to practical approaches
• Using Matlab for data analysis
• Understanding statistics using practical approaches
• Computer-based data analysis in Matlab
• Simple coding for metabolomics
• +++Contents of teaching Basics of Membrane Biophysics:+++ - see LS4131

Qualification-goals/Competencies:

• The students understand the methods using in metabolomics research
• They understand basic metabolic mechanisms and their importance for drug research
• They understand the statistical methods used in metabolic analyses
• They have developed a basic understanding of methods used to model metabolic networks
• Students have a basic theoretical understanding of cryo-EM and can weigh up the method against other structural biology methods
• The students know which basic experimental steps are required to determine a protein structure.
• Students have gained initial experience in the processing of cryo-EM data
• Qualification-goals/Competencies Basics of Membrane Biophysics see LS4131

Grading through:

• written exam

Responsible for this module:

• Prof. Dr. rer. nat. Ulrich Günther

Teacher:

• Institute of Chemistry and Metabolomics
• Institute of Biochemistry
• Prof. Dr. Thomas Krey
• Prof. Dr. rer. nat. Ulrich Günther
• PD Dr. rer. nat. Guido Hansen

Literature:

• new papers :

Language:

• English, except in case of only German-speaking participants

Notes:

Last Updated:

31.03.2025