Module LS2810-KP04

Duration

1 Semester

Turnus of offer

each summer semester

Credit points

4

Course of studies, specific fields and terms:

• Bachelor Molecular Life Science 2024, optional subject, life sciences
• Bachelor MLS 2016, optional subject, life sciences
• Bachelor MLS 2018, optional subject, life sciences

Classes and lectures:

• Stem Cell Technology Seminar (practical course, 2 SWS)
• Stem Cell Technology Seminar (seminar, 1 SWS)

Workload:

• 45 hours in-classroom work
• 75 hours private studies

Contents of teaching:

• This seminar, offered in the cell culture labs at the Institute of Neurogenetics, provides inside and practical experience of the generation/application of human induced pluripotent stem cells (iPSC): Seminar:
• Basics of stem cell and developmental biology
• Basics of cell culture techniques
• Introduction to the reprogramming of somatic cells into iPSCsBasics of cell culture techniques
• Introduction to the differentiation of stem cells / Application for disease modeling
• Presentation of CRISPR/Cas9 technology as a tool to genome edit iPSCs
• Practical part:
• Cultivation of iPSCs (Freezing, thawing, passaging)
• Characterization of iPSCs by immunostaining and live cell assays
• Plating and immunostaining of cortical iPSC-derived neurons followed by confocal microscopy analysis
• Design of gRNAs for CRISPR knockout, CRISPRa, and CRISPRi
• Presentation of a relevant publication regarding iPSC and CRISPR/Cas9 technologies in form of a 10-minute talk

Qualification-goals/Competencies:

• The students know the basics of cell culture using the example of iPSCs
• They can perform an immunostaining of cells and know how to analyze cellular structures by using confocal microscopy software
• They can describe the basics of the new technologies iPSCs and CRISPR/Cas9

Grading through:

• participation in discussions

Responsible for this module:

• Prof. Dr. Philip Seibler

Teacher:

• Prof. Dr. Philip Seibler

Literature:

• :

Language:

• offered only in German

Notes:

Last Updated:

24.07.2023