Module LS3500-KP04

Duration

1 Semester

Turnus of offer

each summer semester

Credit points

4

Course of studies, specific fields and terms:

• Bachelor CLS 2023, optional subject, life sciences
• Bachelor CLS 2016, optional subject, life sciences

Classes and lectures:

• Introduction into Structural Analysis (seminar / exercises, 2 SWS)
• Introduction into Structural Analysis (lecture, 2 SWS)

Workload:

• 60 hours in-classroom work
• 120 hours private studies

Contents of teaching:

• Part A: Protein structure analysis by crystal X-ray diffraction:
• Crystal growth: precipitant and phasediagram
• Crystal morphology: symmetry and space groups
• X-ray diffraction: Bragg's law, reciprocal lattice and the Ewald-sphere construction
• Phase determination: Patterson map and molecular replacement
• Part B: Basic NMR spectroscopy for the investigation of biomolecular structures: Basics of NMR spectroscopy: NMR experiments, Spin systems, the classical vector model
• The nuclear Overhauser effect
• Identification and characterisation of protein-ligand interactions: The transfer nOe, the STD-NMR-experiment, the HSQC experiment, the cross-saturation experiment
• Building blocks for NMR experiments
• Part C: Basics of mass spectrometry: Indroduction and basics
• Ion sources and their fields of application
• Mass analysers
• Structural analysis of biomolecules

Qualification-goals/Competencies:

• The students will acquire basic skills in selected biophysical techniques to analyze the structure and dynamics of biological macromolecules. The emphasis is on understanding the concepts behind these techniques.
• Furthermore, the students will learn how to elucidate the structure of small organic molecules.
• The students will improve their ability in presentation and analysis of complex data.

Grading through:

• written exam

Responsible for this module:

• PD Dr. Alvaro Mallagaray

Teacher:

• Research Center Borstel, Leibniz Lung Center
• Institute of Biochemistry
• Institute of Chemistry and Metabolomics
• PD Dr. Alvaro Mallagaray
• Dr. math. et dis. nat. Jeroen Mesters
• Prof. Dr. rer. nat. Karsten Seeger
• PD Dr. Dominik Schwudke

Literature:

• : Will be adapted to the current conditions and stated in the lecture. See also in the corresponding scripts
• Teil B: Horst Friebolin : Ein- und zweidimensionale NMR-Spektroskopie. Eine Einführung Wiley-VCH
• Alexander Mc Pherson : Introduction to Macromolecular Crystallography 1st edition, 2003, Wiley

Language:

• German and English skills required

Notes:

Last Updated:

20.03.2026