Module ME4270-KP04

Duration

1 Semester

Turnus of offer

every summer semester

Credit points

4

Course of studies, specific fields and terms:

• Master MES 2020, optional subject, medical engineering science
• Master MES 2014, optional subject, medical engineering science

Classes and lectures:

• ME4270-Ü: Diffraction, Resolution and Superresolution (exercise, 1 SWS)
• ME4270-V: Diffraction, Resolution and Superresolution (lecture, 2 SWS)

Workload:

• 30 hours oral presentation (including preparation)
• 45 hours private studies
• 15 hours group work
• 30 hours in-classroom work

Contents of teaching:

• Wave optical properties of light
• Gaussian beams and diffraction phenomena
• Wavefront measurement and beam characterization
• Aberration compensation and adaptive optics
• Basics of Fourier optics
• Determination of the resolution limit of a microscope according to Abbe
• Experimental visualization of the Gouy phase
• Axial resolution enhancement in confocal microscopes
• Basics of super-resolution microscopy

Qualification-goals/Competencies:

• The overall goal is to provide a practical understanding of wave-optical light phenomena and their influence on microscopic resolution. Furthermore, super-resolution microscopy methods are discussed and made understandable experimentally:
• Students explore the wave-optical properties of light, from Gaussian ray optics to the diffraction of light at interfaces and in optical imaging.
• Students will learn about wavefront aberrations, their origin and quantification, and adaptive methods to compensate for them.
• Fourier optics fundamentals and their relation to modern microscopy are taught in a practical manner.
• Fundamentals of modern super-resolution microscopy will be taught. The underlying ideas of resolution enhancement beyond the diffraction limit are exemplified in the laboratory.
• Students acquire technical, social and communication skills through group discussion of complex technical problems.
• Within the framework of short presentations, you will learn to work out complex topics in a team and to present them in a compact way.

Grading through:

• scientific presentation
• continuous, successful participation in course
• protocols

Responsible for this module:

• Prof. Dr.-Ing. Maik Rahlves

Teacher:

• Institute of Biomedical Optics
• Prof. Dr.-Ing. Maik Rahlves
• Prof. Dr. rer. nat. Sebastian Karpf

Literature:

• B. E. A. Saleh, M.C. Teich : Grundlagen der Photonik John Wiley & Sons, USA
• J.W. Goodman : Fourier Optics Roberts & Company Publisher, USA
• L. Bergmann, C. Schäfer : Lehrbuch der Experimentalphysik Bd.3, Optik, de Gruyter, Deutschland

Language:

• English, except in case of only German-speaking participants

Last Updated:

31.07.2023