Module LS4022-KP04

Duration

1 Semester

Turnus of offer

each winter semester

Credit points

4

Course of studies, specific fields and terms:

• Master MES 2020, optional subject, mathematics / natural sciences

Classes and lectures:

• Seminar Single molecule methods (seminar, 1 SWS)
• Single molecule methods (lecture, 2 SWS)

Workload:

• 45 hours in-classroom work
• 55 hours private studies and exercises
• 20 hours exam preparation

Contents of teaching:

• Physical Fundamentals of Fluorescence
• Photophysics
• Methods of single molecule fluorescence microscopy
• Protein labeling and immobilization
• Fluorescence Resonance Energy Transfer (FRET)
• Enzyme activity with single molecules
• Single molecule protein folding
• Physical basis of optical tweezers
• Protein folding with optical tweezers

Qualification-goals/Competencies:

• Students will be able to explain and apply the physical principles of fluorescence.
• They can explain and apply the basics of photophysics and photochemistry.
• They can select suitable detection methods for single molecules.
• They can select appropriate protein labelling methods.
• They can analyze and critically evaluate the data obtained.
• They have an overview of current research in the field of fluorescence spectroscopy of individual biomolecules.
• They have an insight into the structures of the research landscape.

Grading through:

• Written or oral exam as announced by the examiner

Requires:

• Introduction into Biophysics (LS2200-KP04, LS2200)

Responsible for this module:

• Prof. Dr. rer. nat. Christian Hübner

Teacher:

• Institute of Physics
• Prof. Dr. rer. nat. Christian Hübner

Literature:

• Lakowicz, Joseph R. : Principles of Fluorescence Spectroscopy ISBN 978-0-387-46312-4
• Markus Sauer, Johan Hofkens, Jörg Enderlein : Handbook of Fluorescence Spectroscopy and Imaging: From Ensemble to Single Molecules ISBN: 978-3-527-31669-4

Language:

• English, except in case of only German-speaking participants

Notes:

Last Updated:

06.12.2019