Module ME4220-KP04, ME4220

Duration

1 Semester

Turnus of offer

each winter semester

Credit points

4

Course of studies, specific fields and terms:

• Master MES 2020, optional subject, medical engineering science
• Master MES 2014, optional subject, medical engineering science

Classes and lectures:

• Microscopic optical techniques (practical course, 1 SWS)
• Microscopic optical techniques (lecture, 2 SWS)

Workload:

• 15 hours group work
• 30 hours in-classroom work
• 30 hours private studies
• 45 hours written report

Contents of teaching:

• V: Geometrical optics, wave optics & Fourier optics of microscopic imaging
• P: Numerical aperture and resolution, Köhler illumination, interference and speckle, Michelson interferometer
• V: Phase contrast and differential interference contrast for visualization of phase objects, coherent filtering
• P: Diffraction & optical Fourier transformation, coherent filtering, phase contrast, DIC,
• V: Photophysics of organic dyes, fluorescence microscopy, nonlinear optics
• P: Fluorescence spectroscopy, filter sets, dyes, photobleaching
• V: Confocal & multiphoton laser scanning microscopy, Resolution beyond Abbe limit
• P: Confocal laser scanning and 2-photon microscope: preparation and imaging of various specimens
• V/P: Cell surgery with focused laser pulses and via nano-particlesd, laser-based specimen transport
• V/P: Wave front measurement and adaptive optics

Qualification-goals/Competencies:

• The students have gained a profound understanding of microscopic optical techniques and are able to apply it and to relate it to new applications.
• They can assess the function of optical components and are able to select them and to combine them for practical applications within a research or development project.
• The students are able to design complex optical setups and to arrange them on an optical bench.
• They have the professional, social and communication competencies to discuss scientific problems in tutorial groups and to implement potential solutions.

Grading through:

• Written report

Responsible for this module:

• Prof. Dr. rer. nat. Robert Huber

Teacher:

• Institute of Anatomy
• Institute of Biomedical Optics
• Dr. rer. nat. Norbert Linz
• Prof. Dr. med. Peter König
• Prof. Dr. rer. nat. Gereon Hüttmann

Literature:

• Douglas B. Murphy : Fundamentals of Light Microscopy and Electronic Imaging Wiley-Liss, 2001
• Christian Linkenheld : Pfad durch die Lichtmikroskopie /li>
• Barry R. Masters, Peter T.C. So (Hrsg) : Handbook of Biomedical Nonlinear Optical Microscopy Oxford University Press, 2008
• Jerome Mertz : Introduction to Optical Microscopy Roberts and Company, Colorado, 2011 (advanced)
• Joseph W. Goodman : Introduction to Fourier Optics 3rd Ed.. Roberts and Company, Colorado, 2005 (advanced)

Language:

• offered only in German

Notes:

Last Updated:

28.03.2025