Module LS4020 C-MIW
Module part: Single molecule methods (EinzelStrT)
Duration
1 Semester
Turnus of offer
each winter semester
Credit points
4
Course of studies, specific fields and terms:
- Master MES 2020, module part, mathematics / natural sciences
- Master Entrepreneurship in Digital Technologies 2020, module part, Module part
- Master Entrepreneurship in Digital Technologies 2014, module part, Module part
- Master MES 2014, module part, mathematics / natural sciences
Classes and lectures:
- Single molecule methods (lecture, 2 SWS)
- Seminar Single molecule methods (seminar, 1 SWS)
Workload:
- 20 hours exam preparation
- 55 hours private studies and exercises
- 45 hours in-classroom work
Contents of teaching:
- Physical Fundamentals of Fluorescence
- Photophysics
- Methods of single molecule fluorescence microscopy
- Protein labeling and immobilization
- Fluorescence Resonance Energy Transfer (FRET)
- Enzyme activity with single molecules
- Single molecule protein folding
- Physical basis of optical tweezers
- Protein folding with optical tweezers
Qualification-goals/Competencies:
- Students will be able to explain and apply the physical principles of fluorescence.
- They can explain and apply the basics of photophysics and photochemistry.
- They can select suitable detection methods for single molecules.
- They can select appropriate protein labelling methods.
- They can analyze and critically evaluate the data obtained.
- They have an overview of current research in the field of fluorescence spectroscopy of individual biomolecules.
- They have an insight into the structures of the research landscape.
Grading through:
- Written or oral exam as announced by the examiner
Responsible for this module:
- Siehe Hauptmodul
Literature:
- Lakowicz, Joseph R. : Principles of Fluorescence Spectroscopy ISBN 978-0-387-46312-4
- Markus Sauer, Johan Hofkens, Jörg Enderlein : Handbook of Fluorescence Spectroscopy and Imaging: From Ensemble to Single Molecules ISBN: 978-3-527-31669-4
Language:
- English, except in case of only German-speaking participants
Notes:
Prerequisites for attending the module:- None (The competences of the required modules are required for this module, but the modules are not a prerequisite for admission).
Prerequisites for the exam:
- Preliminary examinations can be determined at the beginning of the semester. If preliminary work has been defined, it must have been completed and positively assessed before the initial examination.
Last Updated:
06.12.2019